human pluripotent stem cell functional identification kit (R&D Systems)

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R&D Systems human pluripotent stem cell functional identification kit
Human Pluripotent Stem Cell Functional Identification Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+pluripotent+stem+cell+functional+identification+kit/Human+Pluripotent+Stem+Cell+Functional+Identification+Kit/pm41946259-71-6-13
Average 96 stars, based on 170 article reviews

human pluripotent stem cell functional identification kit - by Bioz Stars, 2026-09

96/100 stars

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Functional Assay:

Article Title: Generation of two induced pluripotent stem cell lines from female patients harboring MYH7 mutations with hypertrophic cardiomyopathy
Article Snippet: .. Ectoderm differentiation was performed by following the instructions of the commercial Human Pluripotent Stem Cell Functional Identification Kit (Cat #SC027B, R&D Systems). .. Endoderm differentiation was performed using the STEMdiffTM Definitive Endoderm Kit (Cat #05110, STEMCELL Technologies).

Article Title: Generation and characterization of two human induced pluripotent stem cell lines from myotonic dystrophy type 1 patients
Article Snippet: .. Ectoderm was generated using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems #SC027B). ..

Article Title: Collagen hydrogel tube microbioreactors for cell and tissue manufacturing
Article Snippet: .. For trilineage differentiation, hPSCs were directed into the three germ layers using the Human pluripotent stem cell functional identification kit (R&D Systems), following the product instructions. ..

Article Title: Modeling spastic paraplegia 4 with corticospinal motor neuron-enriched cortical organoids reveals genotype-phenotype and HDAC6-targetable pathology
Article Snippet: The lines were validated for their pluripotency by immunostaining for OCT4, SSEA-4, SOX2, and TRA1-60 (Thermo Fisher Scientific, A24881). .. Germ layer differentiation was conducted using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) and G-banded karyotype and mutation analysis was also carried out to confirm CRISPR-Cas9 based mutation. .. All hiPSC lines were cultured in 5% CO 2 at 37°C in mTeSR Plus (Stem Cell Technologies, 100–0276) on Matrigel GFR Basement Membrane Matrix (Corning, 356231) as previously reported.

Article Title: Generation of two induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying MYBPC3 mutations.
Article Snippet: .. For ectoderm differentiation, we used the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, Cat #SC027B). .. For mesoderm differentiation, we introduced 6 μM CHIR-99021 (Selleck Chemicals, #S2924) into RPMI medium supplemented with B27 supplement minus insulin (Gibco, #11875–085 and #A18956-01) and sustained in culture for 48 h. For endoderm differentiation, we used the StemDiffTM Definitive Endoderm Differentiation Kit (STEMCELLTM Technologies, Cat #05110).

Article Title: Generation of an induced pluripotent stem cell line CGOi001-A from a patient with hereditary thrombocytopenia and a germline ANKRD26 mutation.
Article Snippet: .. Lastly, we used the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, Cat #SC027B) to perform a directed differentiation into ectoderm, endoderm, and mesoderm to confirm CGOi001-A iPSCs remained functionally pluripotent (Fig. 1I) at passage 12. ..

Article Title: Generation of induced pluripotent stem cell lines from patients with Emery-Dreifuss muscular dystrophy.
Article Snippet: .. Ectoderm differentiation was carried out using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, Cat #SC027B). .. Mesoderm differentiation was achieved by culturing iPSCs in RPMI medium containing B27 Minus Insulin supplement (Gibco, Cat #11875-085 and Cat #A18956-01) and 6 μM CHIR99021 (Selleck Chemicals, Cat #S2924) for 48 h.

other:

Article Title: Modeling spastic paraplegia 4 with corticospinal motor neuron-enriched cortical organoids reveals genotype-phenotype and HDAC6-targetable pathology.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SOX2 R&D Systems Cat# AF2018; RRID:AB_355110 Neun Abcam Cat# ab104224; RRID:AB_10711040 MAP2 Novus Biologicals NB300-213 Cat# NB300-213; RRID:AB_2138178 βIII- Tubulin BioLegend Cat# 802001; RRID:AB_2564645 CTIP2 Abcam Cat# ab18465; RRID:AB_2064130 GFAP Abcam Cat# ab53554; RRID:AB_880202 SMI312 BioLegend Cat# 837904; RRID:AB_2566782 L1CAM BioLegend Cat# 371607; RRID:AB_2734424 vGlut1 Synaptic Systems Cat# 135 302; RRID:AB_887877 Synaptophysin Proteintech Proteintech Cat# 60191-1-Ig; RRID:AB_10915965 SMI32 BioLegend Cat# 801701; RRID:AB_2564642 Acetylated Tubulin Sigma-Aldrich Cat# T6793; RRID:AB_477585 HDAC6 Cell Signaling Technology Cat# 7612; RRID:AB_10889735 βIII- Tubulin Abcam Cat# ab7751; RRID:AB_306045 GAPDH Abcam Cat# ab8245; RRID:AB_2107448 Spastin Abcam Cat# ab77144; RRID:AB_1524436 Anti-Flag Proteintech Cat# 20543-1-AP; RRID:AB_11232216 Anti-Phosphoserine/threonine PhosphoSolutions Cat# PP2551; RRID:AB_1184778 Anti-HDAC6 Affinity Biosciences Cat# AF6485; RRID:AB_2835165 Anti-mCherry Abcam Cat# ab205402; RRID:AB_2722769 Chemicals, peptides, and recombinant proteins MTeSR TM Plus Stem Cell Technologies 100–0276 DMEM-F12 Gibco 11330032 ReLeSR TM Stem Cell Technologies 100–0483 ROCK inhibitor Y-27632 Tocris 1254 Dorsomorphin Sigma C956T21 SB431542 Tocris 1614 Neurobasal A Thermo Fisher 10-888- 022 B-27 without Vitamin A Thermo Fisher 12587010 Glutamax Thermo Fisher 35- 050-061 EGF PeproTech AF-100-15 bFGF Peprotech 100-18B Cyclopamine Fisher Scientific AAJ61528MB B-27 Plus Thermo Fisher Scientific A3582801 Normocin InvivoGen ant-nr-05 BDNF PeproTech 450–02 NT3 PeproTech 450–03 StemPro Accutase TM Cell Dissociation Reagent Thermo Fisher Scientific A1110501 DNAase I Thermo Fisher Scientific EN0521 BrainPhys Neuronal Medium Stem Cell Technologies 05790 Tubastatin A Selleckchem S8049 Paraformaldehyde Electron Microscopy Sciences 19202 Fetal bovine serum Novus Biologicals S11150 (Continued on next page) 16 Cell Reports 45, 117036, March 24, 2026

Generated:

Mutagenesis:

Article Title: Modeling spastic paraplegia 4 with corticospinal motor neuron-enriched cortical organoids reveals genotype-phenotype and HDAC6-targetable pathology
Article Snippet: The lines were validated for their pluripotency by immunostaining for OCT4, SSEA-4, SOX2, and TRA1-60 (Thermo Fisher Scientific, A24881). .. Germ layer differentiation was conducted using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) and G-banded karyotype and mutation analysis was also carried out to confirm CRISPR-Cas9 based mutation. .. All hiPSC lines were cultured in 5% CO 2 at 37°C in mTeSR Plus (Stem Cell Technologies, 100–0276) on Matrigel GFR Basement Membrane Matrix (Corning, 356231) as previously reported.

CRISPR:

Article Title: Modeling spastic paraplegia 4 with corticospinal motor neuron-enriched cortical organoids reveals genotype-phenotype and HDAC6-targetable pathology
Article Snippet: The lines were validated for their pluripotency by immunostaining for OCT4, SSEA-4, SOX2, and TRA1-60 (Thermo Fisher Scientific, A24881). .. Germ layer differentiation was conducted using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) and G-banded karyotype and mutation analysis was also carried out to confirm CRISPR-Cas9 based mutation. .. All hiPSC lines were cultured in 5% CO 2 at 37°C in mTeSR Plus (Stem Cell Technologies, 100–0276) on Matrigel GFR Basement Membrane Matrix (Corning, 356231) as previously reported.

Review